RNA

Part:BBa_K2923017:Design

Designed by: Kateryna LEN   Group: iGEM19_Strasbourg   (2019-10-13)


Two MS2 stem-loops


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains differents restrictions sites: BspMI, PstI, SalI, AccI, HincII, XmaI.


Source

Escherichia phage MS2 [Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46]

References

Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46